Assay:	_A_20200302-LUM
Short Name:	20200302-LUM
Assay Class:	WET
Assay Type:	LUM
Title:	Luciferase Assay US1.7.X and US3.X pieces
Description:	Analysis of new US pieces with three recognition sites for gRNA1 DFR (US3.X) and US pieces with the recognition site in different positions (US1.7.1-US1.7.4), as well as another US part with the recognition site of another gRNA (US1.7.5, for gRNA4 from NOS promoter).
pISA Assay creation date:	2020-02-03
pISA Assay creator:	ElenaMG
Lab manager:	DO
Operator:	ElenaMG
Link to SynBio DM:	https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703
Link to protocols:	../protocols/LUCIFERASE
Additional Notes:	
Phenodata:	../../phenodata_Luciferase_20211222.txt
Featuredata:	
Plate ID:	05
DNA introduction system:	agrobacteria mediated transient transformation
Date of transformation/transfection:	2020-01-29
Treatment:	none
Date of treatment:	2021-11-20
Date of sampling:	2021-11-20
Date of luminescence assay start:	2020-02-03
Time of luminescence assay start:	12:00
Luminometer:	GloMax Multidetection system (Promega)
Protocol (luminometer setup):	Luminiscence 3x read
Time [min] between two measurements (luminometer setup):	2
Integration time [ms] (luminometer setup):	10000
Temperature (luminometer) at start of the experiment [degrees Celsius]:	24
Temperature (luminometer) at the end of the experiment [degrees Celsius]:	24
Time between start of sampling and start of experiment [min]:	60
Silencing supressor added:	p19
Notes:	
Data: