Assay:	_A_20200219-LUM
Short Name:	20200219-LUM
Assay Class:	WET
Assay Type:	LUM
Title:	Luciferase assay of different A1 random sequences
Description:	With the aim of making three promoters as different as possible for the moth pheromone enzymes, three A1 pieces containing random sequences withous any promoter signal were assembled and tested using luciferase assay.
pISA Assay creation date:	2020-02-19
pISA Assay creator:	ElenaMG
Lab manager:	DO
Operator:	ElenaMG
Link to SynBio DM:	https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703
Link to protocols:	../protocols/LUCIFERASE
Additional Notes:	
Phenodata:	../../phenodata_Luciferase_20211222.txt
Featuredata:	
Plate ID:	04
DNA introduction system:	agrobacteria mediated transient transformation
Date of transformation/transfection:	2020-02-14
Treatment:	none
Date of treatment:	2020-02-14
Date of sampling:	2020-02-19
Date of luminescence assay start:	2020-02-19
Time of luminescence assay start:	12:00
Luminometer:	GloMax Multidetection system (Promega)
Protocol (luminometer setup):	Luminiscence 3x read
Time [min] between two measurements (luminometer setup):	2
Integration time [ms] (luminometer setup):	10000
Temperature (luminometer) at start of the experiment [degrees Celsius]:	24
Temperature (luminometer) at the end of the experiment [degrees Celsius]:	24
Time between start of sampling and start of experiment [min]:	60
Silencing supressor added:	p19
Notes:	
Data: