Assay:	_A_20190208-LUM
Short Name:	20190208-LUM
Assay Class:	WET
Assay Type:	LUM
Title:	Luciferase assay first trial 
Description:	Promoter series containing random sequences and the recognition sequence for guideRNA1 DFR (P1.X series) fused to the developed core promoter minDFR and to luciferase for measuring the expression in the presence or absence of such guideRNA1 DFR. A promoter containing the target sequence in a different position (P1.7.1) and a promoter with the target sequence repeated twice (P2.1) were also assessed.
pISA Assay creation date:	2019-02-08
pISA Assay creator:	ElenaMG
Lab manager:	DO
Operator:	ElenaMG
Link to SynBio DM:	https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703
Link to protocols:	../protocols/LUCIFERASE
Additional Notes:	
Phenodata:	../../phenodata_Luciferase_20211222.txt
Featuredata:	
Plate ID:	01
DNA introduction system:	agrobacteria mediated transient transformation
Date of transformation/transfection:	2019-02-04
Treatment:	none
Date of treatment:	2019-02-04
Date of sampling:	2019-02-08
Date of luminescence assay start:	2019-02-08
Time of luminescence assay start:	12:00
Luminometer:	GloMax Multidetection system (Promega)
Protocol (luminometer setup):	Luminiscence 3x read
Time [min] between two measurements (luminometer setup):	2
Integration time [ms] (luminometer setup):	10000
Temperature (luminometer) at start of the experiment [degrees Celsius]:	24
Temperature (luminometer) at the end of the experiment [degrees Celsius]:	24
Time between start of sampling and start of experiment [min]:	60
Silencing supressor added:	p19
Notes:	
Data: