Investigation:	_I_T11_dCas9SynProm
Short Name:	T11_dCas9SynProm
Title:	Development of synthetic promoters inducible by dCasEV2.1 system
Description:	The aim of this work is to design a range of synthetic promoters with negligible basal expression that are activated by using the "dead" Cas9 activation system developed in our lab (dCasEV2.1, Selma et al., 2019). Such tool will then be used for creating synthetic regulatory cascades where the expression of the genes for the pheromone biosynthesis can be controlled by a single dCas9 gene at custom expression levels. By adding as much variability as possible to each synthetic promoter, issues arising from the use of similar promoters very close to each other's will be avoided. We also aim to have synthetic promoters with different strength to further modulate the biosynthetic pathways.
Phenodata:	./phenodata_Luciferase_20211222.txt
Principal investigator:	Diego Orzaez
License:	Creative Commons Attribution 4.0
Sharing permission:	Private
Upload to FAIRDOMHub:	Yes