This model has been created with the help of the SABIO-RK Database (http://sabio.h-its.org/) (c) 2005-2016 HITS gGmbH http://www.h-its.org


To cite SABIO-RK Database, please use "http://www.ncbi.nlm.nih.gov/pubmed/22102587"
SABIO-RK - database for biochemical reaction kinetics. Wittig U, Kania R, Golebiewski M, Rey M, Shi L, Jong L, Algaa E, Weidemann A, Sauer-Danzwith H, Mir S, Krebs O, Bittkowski M, Wetsch E, Rojas I, Mueller W. Nucleic Acids Res. 2012;40(Database issue)790-6 E kcat Km S kcat_Km kcat E S Km S E kcat_Km S Km kcat kcat E S Km S S kcat kcat_Km Km E kcat E S Km S kcat E Km kcat_Km S kcat E S Km S kcat_Km E S kcat Km kcat E S Km S kcat Km S E kcat_Km kcat E S Km S Km B Act Km Km Act A Km kcat E kcat_Km E kcat A Km A kcat_Km A Km E kcat E kcat A Km A kcat E Km kcat_Km A E kcat A Km A kcat_Km Km kcat E A E kcat A Km A kcat A E Km kcat_Km E kcat A Km A kcat_Km A Km E kcat E kcat A Km A Km kcat A kcat_Km E E kcat A Km A A kcat kcat_Km E Km E kcat A Km A kcat E Km A kcat_Km E kcat A Km A kcat kcat_Km Km E A E kcat A Km A Km A Vmax Act M Vmax A Km A M Km Vmax A Act Vmax A Km A A Km M Vmax Vmax A Km A Km Vmax M A Vmax A Km A A M Vmax Km Vmax A Km A Vmax A Km A Vmax Km A Km Vmax A Km Vmax Vmax A Km A Vmax Km A Vmax A Km A E kcat kcat_KmB kcat_KmA A KmA B KmB E kcat B KmB B A KmA A A E Km kcat kcat_Km E kcat A Km A B kcat_KmB kcat_KmA KmB kcat KmA A E E kcat B KmB B A KmA A KmB A B E kcat kcat_KmA KmA kcat_KmB E kcat B KmB B A KmA A KmB B kcat_KmA kcat_KmB KmA kcat A E E kcat B KmB B A KmA A A B KmB KmA E kcat_KmB kcat_KmA kcat E kcat B KmB B A KmA A KmA kcat_KmA E kcat kcat_KmB KmB A B E kcat B KmB B A KmA A E kcat A kcat_Km Km E kcat A Km A B Km kcat_Km A Activity Activity kcat_Km Km B A Activity kcat_Km B A Km Activity Km A B A Activity B Km A B Km Activity A B Activity Km Km A Activity B kcat_Km B A Km Activity Activity A Km kcat_Km B Activity A kcat_Km Km B kcat_Km B A Activity Km Ki Vmax E Km S Vmax S S Km S Vmax Km Vmax S S Km S Km Vmax Vmax S S Km Vmax A Km C Ki I I Ki C I A Ki I Ki A Ki A I Km A Km A Km A Vmax Vmax A Km A A Km C Km Km Km S Km Km S Vmax A Km A Km Vmax Spec_Act Spec_Act Km A Vmax A Km Spec_Act Vmax Vmax Km A Spec_Act IC_50 A IC_50 A IC_50 A A IC_50 Km C Km C C Km C Km Km C C Km Km C C Km Km Vmax S Km Vmax S S Km S Vmax Km Vmax S S Km Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Cofactor Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Inhibitor Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Cofactor Modifier-Catalyst Modifier-Inhibitor Modifier-Catalyst Modifier-Inhibitor Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst Modifier-Catalyst 26292 8.0 37.0 °C 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U pyrovate kinase, 66,7 U lactate dehydrogenase / ml KL_26292 ENZ_148458_Cell REAC_0_kcat REAC_0_Km_SPC_1661_Cell SPC_1661_Cell REAC_0_kcat_Km_SPC_1661_Cell 26290 8.0 37.0 °C 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U pyrovate kinase, 66,7 U lactate dehydrogenase / ml KL_26290 ENZ_148448_Cell REAC_1_kcat_Km_SPC_1661_Cell SPC_1661_Cell REAC_1_Km_SPC_1661_Cell REAC_1_kcat 26294 8.0 37.0 °C 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U pyrovate kinase, 66,7 U lactate dehydrogenase / ml KL_26294 SPC_1661_Cell REAC_2_kcat REAC_2_kcat_Km_SPC_1661_Cell REAC_2_Km_SPC_1661_Cell ENZ_148468_Cell 26291 8.0 37.0 °C 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U pyrovate kinase, 66,7 U lactate dehydrogenase / ml KL_26291 REAC_3_kcat ENZ_148453_Cell REAC_3_Km_SPC_34_Cell REAC_3_kcat_Km_SPC_34_Cell SPC_34_Cell 26295 8.0 37.0 °C 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U pyrovate kinase, 66,7 U lactate dehydrogenase / ml KL_26295 REAC_4_kcat_Km_SPC_34_Cell ENZ_148473_Cell SPC_34_Cell REAC_4_kcat REAC_4_Km_SPC_34_Cell 26293 8.0 37.0 °C 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U pyrovate kinase, 66,7 U lactate dehydrogenase / ml KL_26293 REAC_5_kcat REAC_5_Km_SPC_34_Cell SPC_34_Cell ENZ_148463_Cell REAC_5_kcat_Km_SPC_34_Cell 44091 37.0 °C 7.0 200 mM Sodium phosphate KL_44091 REAC_6_Km_SPC_1661_Cell SPC_1262_Cell 45367 8.0 coupled assay with pyruvate kinase and lactate dehydrogenase KL_45367 REAC_7_Act REAC_7_Km_SPC_1661_Cell 45366 7.5 coupled assay with pyruvate kinase and lactate dehydrogenase KL_45366 REAC_8_Km_SPC_1661_Cell REAC_8_Act 45393 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45393 SPC_3919_Cell REAC_9_Km_SPC_3919_Cell REAC_9_kcat ENZ_257582_Cell REAC_9_kcat_Km_SPC_3919_Cell 45394 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45394 REAC_10_kcat_Km_SPC_3919_Cell SPC_3919_Cell REAC_10_Km_SPC_3919_Cell ENZ_257585_Cell REAC_10_kcat 45397 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45397 REAC_11_kcat ENZ_257594_Cell REAC_11_Km_SPC_3919_Cell REAC_11_kcat_Km_SPC_3919_Cell SPC_3919_Cell 45399 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45399 REAC_12_kcat_Km_SPC_3919_Cell REAC_12_Km_SPC_3919_Cell REAC_12_kcat ENZ_257600_Cell SPC_3919_Cell 45395 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45395 REAC_13_kcat SPC_3919_Cell ENZ_257588_Cell REAC_13_Km_SPC_3919_Cell REAC_13_kcat_Km_SPC_3919_Cell 45396 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45396 REAC_14_kcat_Km_SPC_3919_Cell SPC_3919_Cell REAC_14_Km_SPC_3919_Cell ENZ_257591_Cell REAC_14_kcat 45398 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45398 REAC_15_Km_SPC_3919_Cell REAC_15_kcat SPC_3919_Cell REAC_15_kcat_Km_SPC_3919_Cell ENZ_257597_Cell 45402 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45402 SPC_3919_Cell REAC_16_kcat REAC_16_kcat_Km_SPC_3919_Cell ENZ_257609_Cell REAC_16_Km_SPC_3919_Cell 45401 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45401 REAC_17_kcat ENZ_257606_Cell REAC_17_Km_SPC_3919_Cell SPC_3919_Cell REAC_17_kcat_Km_SPC_3919_Cell 45400 37.0 °C 8.8 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase KL_45400 REAC_18_kcat REAC_18_kcat_Km_SPC_3919_Cell REAC_18_Km_SPC_3919_Cell ENZ_257603_Cell SPC_3919_Cell 46263 37.0 °C 8.7 0.4 M Glycine, 0.6 mM DTT, 0.18 mg/ml BSA KL_46263 REAC_19_Km_SPC_1283_Cell SPC_1283_Cell REAC_19_Vmax REAC_19_Act SPC_37_Cell 46264 37.0 °C 8.7 0.4 M Glycine, 0.6 mM DTT, 0.18 mg/ml BSA KL_46264 SPC_37_Cell REAC_20_Km_SPC_1283_Cell REAC_20_Vmax SPC_1283_Cell REAC_20_Act 46260 37.0 °C 8.7 0.04 M Glycine KL_46260 SPC_1283_Cell REAC_21_Km_SPC_1283_Cell SPC_37_Cell REAC_21_Vmax 46262 37.0 °C 8.7 0.04 M Glycine KL_46262 REAC_22_Km_SPC_1283_Cell REAC_22_Vmax SPC_37_Cell SPC_1283_Cell 46261 37.0 °C 8.7 0.04 M Glycine KL_46261 SPC_1283_Cell SPC_37_Cell REAC_23_Vmax REAC_23_Km_SPC_1283_Cell 46560 4.6 37.0 °C 0.1 M Sodium citrate KL_46560 REAC_24_Vmax SPC_28371_Cell REAC_24_Km_SPC_28371_Cell 46558 4.6 37.0 °C 0.1 M Sodium citrate KL_46558 SPC_28371_Cell REAC_25_Vmax REAC_25_Km_SPC_28371_Cell 46559 4.6 37.0 °C 0.1 M Sodium citrate KL_46559 SPC_28371_Cell REAC_26_Km_SPC_28371_Cell REAC_26_Vmax 47202 4.4 20.0 °C 0.15 M Citrate/phosphate KL_47202 SPC_28371_Cell REAC_27_Km_SPC_28371_Cell REAC_27_Vmax 47201 4.4 20.0 °C 0.15 M Citrate/phosphate KL_47201 REAC_28_Vmax REAC_28_Km_SPC_28371_Cell SPC_28371_Cell 14786 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14786 ENZ_76993_cytosol REAC_29_kcat REAC_29_kcat_KmB_SPC_34_cytosol REAC_29_kcat_KmA_SPC_1661_cytosol SPC_1661_cytosol REAC_29_KmA_SPC_1661_cytosol SPC_34_cytosol REAC_29_KmB_SPC_34_cytosol 14785 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14785 SPC_1661_cytosol ENZ_76988_cytosol REAC_30_Km_SPC_1661_cytosol REAC_30_kcat REAC_30_kcat_Km_SPC_1661_cytosol 14787 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14787 SPC_34_cytosol REAC_31_kcat_KmB_SPC_34_cytosol REAC_31_kcat_KmA_SPC_1661_cytosol REAC_31_KmB_SPC_34_cytosol REAC_31_kcat REAC_31_KmA_SPC_1661_cytosol SPC_1661_cytosol ENZ_76998_cytosol 14788 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14788 REAC_32_KmB_SPC_34_cytosol SPC_1661_cytosol SPC_34_cytosol ENZ_77003_cytosol REAC_32_kcat REAC_32_kcat_KmA_SPC_1661_cytosol REAC_32_KmA_SPC_1661_cytosol REAC_32_kcat_KmB_SPC_34_cytosol 14791 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14791 REAC_33_KmB_SPC_34_cytosol SPC_34_cytosol REAC_33_kcat_KmA_SPC_1661_cytosol REAC_33_kcat_KmB_SPC_34_cytosol REAC_33_KmA_SPC_1661_cytosol REAC_33_kcat SPC_1661_cytosol ENZ_77018_cytosol 14789 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14789 SPC_1661_cytosol SPC_34_cytosol REAC_34_KmB_SPC_34_cytosol REAC_34_KmA_SPC_1661_cytosol ENZ_77008_cytosol REAC_34_kcat_KmB_SPC_34_cytosol REAC_34_kcat_KmA_SPC_1661_cytosol REAC_34_kcat 14790 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14790 REAC_35_KmA_SPC_1661_cytosol REAC_35_kcat_KmA_SPC_1661_cytosol ENZ_77013_cytosol REAC_35_kcat REAC_35_kcat_KmB_SPC_34_cytosol REAC_35_KmB_SPC_34_cytosol SPC_1661_cytosol SPC_34_cytosol 14792 8.0 37.0 °C 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerrol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyrovate, 7.5 U pyrovate kinase, 10 U lactate dehydrogenase KL_14792 ENZ_77023_cytosol REAC_36_kcat SPC_34_cytosol REAC_36_kcat_Km_SPC_34_cytosol REAC_36_Km_SPC_34_cytosol 33648 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33648 SPC_3918_Cell REAC_37_Km_SPC_1381_Cell REAC_37_kcat_Km_SPC_1381_Cell SPC_1381_Cell REAC_37_Activity 33649 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33649 REAC_38_Activity REAC_38_kcat_Km_SPC_1381_Cell REAC_38_Km_SPC_1381_Cell SPC_3918_Cell SPC_1381_Cell 33647 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33647 REAC_39_Activity REAC_39_kcat_Km_SPC_3918_Cell SPC_1381_Cell SPC_3918_Cell REAC_39_Km_SPC_3918_Cell 33642 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33642 REAC_40_Activity REAC_40_Km_SPC_3918_Cell SPC_3918_Cell SPC_1381_Cell 33643 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33643 SPC_3918_Cell REAC_41_Activity SPC_1381_Cell REAC_41_Km_SPC_3918_Cell 33646 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33646 SPC_3918_Cell SPC_1381_Cell REAC_42_Km_SPC_3918_Cell REAC_42_Activity 33645 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33645 SPC_3918_Cell SPC_1381_Cell REAC_43_Activity REAC_43_Km_SPC_3918_Cell 33644 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33644 REAC_44_Km_SPC_3918_Cell SPC_3918_Cell REAC_44_Activity SPC_1381_Cell 33652 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33652 REAC_45_kcat_Km_SPC_1381_Cell SPC_3918_Cell SPC_1381_Cell REAC_45_Km_SPC_1381_Cell REAC_45_Activity 33651 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33651 REAC_46_Activity SPC_1381_Cell REAC_46_Km_SPC_1381_Cell REAC_46_kcat_Km_SPC_1381_Cell SPC_3918_Cell 33653 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33653 REAC_47_Activity SPC_1381_Cell REAC_47_kcat_Km_SPC_1381_Cell REAC_47_Km_SPC_1381_Cell SPC_3918_Cell 33650 8.7 37.0 °C 0.1 M GLY-GLY, 5 mM Dithiothreitol, 5 µM Glucose 1,6-diphosphate, 5 µM MgCl2, 0.8 mM NADP, 0.15 µg/ml Glucose-6-phosphate dehydrogenase, 0.25 µg/ml Phosphoglucomutase KL_33650 REAC_48_kcat_Km_SPC_1381_Cell SPC_3918_Cell SPC_1381_Cell REAC_48_Activity REAC_48_Km_SPC_1381_Cell 50494 4.6 37.0 °C 39 mM Na2HPO4, 23 mM citric acid KL_50494 REAC_49_Ki_SPC_5010_lysosome 50491 4.6 37.0 °C 39 mM Na2HPO4, 23 mM citric acid KL_50491 REAC_50_Vmax ENZ_286848_lysosome REAC_50_Km_SPC_28371_lysosome SPC_28371_lysosome 50493 4.6 37.0 °C 39 mM Na2HPO4, 23 mM citric acid KL_50493 SPC_28371_lysosome REAC_51_Vmax REAC_51_Km_SPC_28371_lysosome 50492 4.6 37.0 °C 39 mM Na2HPO4, 23 mM citric acid KL_50492 SPC_28371_lysosome REAC_52_Km_SPC_28371_lysosome REAC_52_Vmax 48027 4.8 37.0 °C Citrate/Phosphate KL_48027 REAC_53_Vmax SPC_28371_Cell REAC_53_Km_SPC_28371_Cell 49017 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49017 SPC_1452_Cell REAC_54_Ki_SPC_6561_Cell SPC_6561_Cell 49018 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49018 SPC_6561_Cell REAC_55_Ki_SPC_6561_Cell SPC_1452_Cell 49019 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49019 SPC_1337_Cell SPC_1298_Cell REAC_56_Ki_SPC_1337_Cell 49013 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49013 SPC_1337_Cell REAC_57_Ki_SPC_1337_Cell SPC_1298_Cell 49014 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49014 REAC_58_Ki_SPC_1337_Cell SPC_1298_Cell SPC_1337_Cell 48034 4.0 37.0 °C 0.025 M Citrate, 0.7% Bovine serum albumin KL_48034 REAC_59_Km_SPC_2789_Cell SPC_2789_Cell 48036 4.0 37.0 °C 0.025 M Citrate, 0.7% Bovine serum albumin KL_48036 REAC_60_Km_SPC_2789_Cell SPC_2789_Cell 48033 4.0 37.0 °C 0.1 M Sodium citrate, 0.7% Bovine serum albumin KL_48033 REAC_61_Km_SPC_28371_Cell SPC_28371_Cell REAC_61_Vmax 48035 4.0 37.0 °C 0.1 M Sodium citrate, 0.7% Bovine serum albumin KL_48035 SPC_28371_Cell REAC_62_Km_SPC_28371_Cell 48994 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_48994 SPC_1452_Cell REAC_63_Km_SPC_1298_Cell 48993 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_48993 REAC_64_Km_SPC_1298_Cell 49004 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49004 REAC_65_Km_SPC_1298_Cell 49063 4.0 37.0 °C 0.1 M sodium citrate KL_49063 SPC_28371_Cell REAC_66_Km_SPC_28371_Cell 49064 4.0 37.0 °C 0.1 M sodium citrate KL_49064 REAC_67_Km_SPC_28371_Cell SPC_28371_Cell 48020 4.6 37.0 °C Citrate/Phosphate KL_48020 REAC_68_Vmax SPC_28371_Cell REAC_68_Km_SPC_28371_Cell 48427 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48427 SPC_28371_Cell REAC_69_Km_SPC_28371_Cell REAC_69_Vmax REAC_69_Spec_Act 48426 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48426 REAC_70_Spec_Act REAC_70_Km_SPC_28371_Cell SPC_28371_Cell REAC_70_Vmax 48425 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48425 SPC_28371_Cell REAC_71_Km_SPC_28371_Cell REAC_71_Spec_Act REAC_71_Vmax 48424 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48424 REAC_72_Vmax REAC_72_Km_SPC_28371_Cell SPC_28371_Cell REAC_72_Spec_Act 48429 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48429 REAC_73_IC_50_SPC_28666_Cell SPC_28371_Cell 48430 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48430 REAC_74_IC_50_SPC_28666_Cell SPC_28371_Cell 48431 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48431 REAC_75_IC_50_SPC_28666_Cell SPC_28371_Cell 48428 4.6 0.1 M Citrate, 75 mM N-Acetylgalactosamine KL_48428 SPC_28371_Cell REAC_76_IC_50_SPC_28666_Cell 48996 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_48996 REAC_77_Km_SPC_1298_Cell SPC_1452_Cell 49001 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49001 REAC_78_Km_SPC_1298_Cell SPC_1452_Cell 49003 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49003 SPC_1452_Cell REAC_79_Km_SPC_1298_Cell 48997 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_48997 SPC_1452_Cell REAC_80_Km_SPC_1298_Cell 48999 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_48999 REAC_81_Km_SPC_1298_Cell SPC_1452_Cell 49002 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49002 SPC_1452_Cell REAC_82_Km_SPC_1298_Cell 48995 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_48995 REAC_83_Km_SPC_1298_Cell SPC_1452_Cell 48998 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_48998 SPC_1452_Cell REAC_84_Km_SPC_1298_Cell 49000 7.6 37.0 °C 0.1 M Tris, 5 mM MgCl2, 0.02 units/ml glucose-6-phosphate dehydrogenase, 0.2 mM NADP KL_49000 REAC_85_Km_SPC_1298_Cell 49159 4.5 37.0 °C 0.017 M citrate phosphate KL_49159 REAC_86_Vmax SPC_28371_Cell REAC_86_Km_SPC_28371_Cell 49160 4.5 37.0 °C 0.017 M citrate phosphate KL_49160 SPC_28371_Cell REAC_87_Vmax REAC_87_Km_SPC_28371_Cell