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Metadata | Values (examples) | ||||||||
Asset Title | PFK Kinetic Data | ||||||||
Uploader | Dawie van Niekerk | ||||||||
Uploader SEEK ID | |||||||||
Project | |||||||||
ASSAY | |||||||||
Assay SEEK ID | 0 | ||||||||
Assay Title | PFK | ||||||||
Assay_type | enzymaticAssay | ||||||||
Technology_type | initial rate experiment | ||||||||
Description | Kinetic characterisation of PFK. Experimental data for enzyme reaction rates. | ||||||||
Experimentalist | Gerald Penkler | ||||||||
Date | |||||||||
SOP | |||||||||
Publication (optional) | |||||||||
Experimental_conditions | |||||||||
Item | temperature | pH | Buffer | Buffer | Buffer | Buffer | |||
Compound (if concentration) | HEPES | MgCl | KCl | NaCl | |||||
Unit | °C | mM | mM | mM | mM | ||||
Start_value (optional) | 37 | 7.17 | 20 | 20 | 10 | 20 | |||
End_value (optional) | |||||||||
Comments | |||||||||
Culture growth | Batch | ||||||||
FACTORS_STUDIED | |||||||||
Item | concentration | concentration | concentration | concentration | |||||
Compound (if concentration) | F6P | ATP | ADP | FBP | |||||
Unit | mM | mM | mM | mM | |||||
Start_value (optional) | 0.234375 | 0 | 0 | 0 | |||||
End_value (optional) | 10 | 5 | 5 | 60 | |||||
SD (optional) | |||||||||
Comments | |||||||||
ENZYME IDENTITY | |||||||||
Enzyme Name (IUBMB) | 6-phosphofructokinase | ||||||||
EC Number | 2.7.1.11 | ||||||||
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20100720 | 0.5 | 0.00488 | 0 | 0 | 0.0415784774898768 | 0.2753822778369225 | 0.150984579750259 | 0.1098243715520404 | 0.02579483570262094 | 0.3921 | 0.38009261477834677 | |
20100720 | 0.5 | 0.00488 | 0 | 0 | 0.0319937336355804 | 0.2753822778369225 | 0.11617934852919852 | 0.3921 | 0.2924729958433533 | |||
20100720 | 0.5 | 0.00488 | 0 | 0 | 0.017158845674571 | 0.2753822778369225 | 0.06230918637666374 | 0.3921 | 0.1568588104413811 | |||
0.5 | 0 | 0 | 0 | 0 | 0.2753822778369225 | 0 | 0 | 0 | 0.3921 | 0 | ||
20100720 | 0.25 | 5 | 0 | 0 | 0.0438636097580931 | 0.2753822925732928 | 0.159282608 | 0.14089915417219143 | 0.010534131444045197 | 0.3921 | 0.4009822927849727 | |
20100720 | 0.25 | 5 | 0 | 0 | 0.0338154313494462 | 0.2753822925732928 | 0.12279450153987752 | 0.3921 | 0.3091261587633469 | |||
20100720 | 0.25 | 5 | 0 | 0 | 0.0387243551851884 | 0.2753822925732928 | 0.14062035297669673 | 0.3921 | 0.3540014334071427 | |||
20100720 | 0.25 | 2.5 | 0 | 0 | 0.026459243431367 | 0.2753822925732928 | 0.09608186199671824 | 0.11972276129936092 | 0.011956721368536018 | 0.3921 | 0.24187904632057136 | |
20100720 | 0.25 | 2.5 | 0 | 0 | 0.0353660933251855 | 0.2753822925732928 | 0.12842544447832585 | 0.3921 | 0.32330164495327895 | |||
20100720 | 0.25 | 2.5 | 0 | 0 | 0.0370832486829168 | 0.2753822925732928 | 0.13466097742303865 | 0.3921 | 0.33899914217725186 | |||
20100720 | 0.25 | 0.625 | 0 | 0 | 0.0624196297706235 | 0.2753822925732928 | 0.22666537193567218 | 0.20317945413461413 | 0.01417941356497894 | 0.3921 | 0.5706134629194698 | |
20100720 | 0.25 | 0.625 | 0 | 0 | 0.0489275753707967 | 0.2753822925732928 | 0.1776714650517141 | 0.3921 | 0.447274892805004 | |||
20100720 | 0.25 | 0.625 | 0 | 0 | 0.0565088665087205 | 0.2753822925732928 | 0.20520152541645614 | 0.3921 | 0.5165798022623063 | |||
20100720 | 0.25 | 0.3125 | 0 | 0 | 0.0329372954140197 | 0.2753822925732928 | 0.11960571286642718 | 0.12080455800704554 | 0.01749720845747492 | 0.3921 | 0.30109861696489226 | |
20100720 | 0.25 | 0.3125 | 0 | 0 | 0.0250916546794733 | 0.2753822925732928 | 0.09111571570200061 | 0.3921 | 0.22937713696232262 | |||
20100720 | 0.25 | 0.3125 | 0 | 0 | 0.0417733583183576 | 0.2753822925732928 | 0.15169224545270882 | 0.3921 | 0.38187411132374244 | |||
20100720 | 0.25 | 0.15625 | 0 | 0 | 0.0378253394781059 | 0.2753822925732928 | 0.13735574326384373 | 0.15402981190470658 | 0.03379112346565418 | 0.3921 | 0.3457830176984553 | |
20100720 | 0.25 | 0.15625 | 0 | 0 | 0.0290936512376455 | 0.2753822925732928 | 0.10564822801706557 | 0.3921 | 0.2659616717159261 | |||
20100720 | 0.25 | 0.15625 | 0 | 0 | 0.0603322574651021 | 0.2753822925732928 | 0.2190854644332105 | 0.3921 | 0.551531601267411 | |||
20100720 | 0.25 | 0.0391 | 0 | 0 | 0.0323401341834368 | 0.2753822925732928 | 0.11743723200659134 | 0.14952986698183945 | 0.05023702308923628 | 0.3921 | 0.29563962531504867 | |
20100720 | 0.25 | 0.0391 | 0 | 0 | 0.0228901098102738 | 0.2753822925732928 | 0.08312121159417546 | 0.3921 | 0.20925155873952875 | |||
20100720 | 0.25 | 0.0391 | 0 | 0 | 0.0683033887392048 | 0.2753822925732928 | 0.2480311573447516 | 0.3921 | 0.6244002619181677 | |||
20100720 | 0.25 | 0.0195 | 0 | 0 | 0.0290354468134082 | 0.2753822925732928 | 0.1054368693865109 | 0.14901956796930294 | 0.024243081873696812 | 0.3921 | 0.26542959185269516 | |
20100720 | 0.25 | 0.0195 | 0 | 0 | 0.0419710257742571 | 0.2753822925732928 | 0.15241003835817274 | 0.3921 | 0.38368110236056496 | |||
20100720 | 0.25 | 0.0195 | 0 | 0 | 0.0521055782093395 | 0.2753822925732928 | 0.18921179616322512 | 0.3921 | 0.47632683065744946 | |||
20100720 | 0.25 | 0.00977 | 0 | 0 | 0.0191573666559246 | 0.2753822925732928 | 0.0695664433501144 | 0.13502871229269833 | 0.04617531063040258 | 0.3921 | 0.1751284230317524 | |
20100720 | 0.25 | 0.00977 | 0 | 0 | 0.0306627793845554 | 0.2753822925732928 | 0.11134622744995314 | 0.3921 | 0.28030596771644567 | |||
20100720 | 0.25 | 0.00977 | 0 | 0 | 0.0617334030226685 | 0.2753822925732928 | 0.22417346607802752 | 0.3921 | 0.5643402725394965 | |||
20100720 | 0.25 | 0.00488 | 0 | 0 | 0.0159365643486446 | 0.2753822925732928 | 0.05787069386243524 | 0.0949403292447107 | 0.028816459530265404 | 0.3921 | 0.14568523080697252 | |
20100720 | 0.25 | 0.00488 | 0 | 0 | 0.0207247339082138 | 0.2753822925732928 | 0.07525804841899168 | 0.3921 | 0.18945662168948021 | |||
20100720 | 0.25 | 0.00488 | 0 | 0 | 0.0417733583183566 | 0.2753822925732928 | 0.15169224545270518 | 0.3921 | 0.38187411132373333 | |||
20100720 | 0.25 | 0 | 0 | 0 | 0 | 0.2753822925732928 | 0 | 0 | 0 | 0.3921 | 0 | |
20100720 | 0.125 | 5 | 0 | 0 | 0.0333930315769804 | 0.2753822925732928 | 0.12126063467967124 | 0.10396564398530357 | 0.008835333045866573 | 0.3921 | 0.30526476135056474 | |
20100720 | 0.125 | 5 | 0 | 0 | 0.0271132887123585 | 0.2753822925732928 | 0.09845690679309863 | 0.3921 | 0.24785804754284935 | |||
20100720 | 0.125 | 5 | 0 | 0 | 0.0253845718792561 | 0.2753822925732928 | 0.09217939048314087 | 0.3921 | 0.23205486027357813 | |||
20100720 | 0.125 | 2.5 | 0 | 0 | 0.0242627935326111 | 0.2753822925732928 | 0.0881058593342692 | 0.07557207475919225 | 0.007474289717455375 | 0.3921 | 0.22180004413065335 | |
20100720 | 0.125 | 2.5 | 0 | 0 | 0.0210282455943137 | 0.2753822925732928 | 0.0763601951229927 | 0.3921 | 0.19223119524716412 | |||
20100720 | 0.125 | 2.5 | 0 | 0 | 0.0171425944781951 | 0.2753822925732928 | 0.06225016982031483 | 0.3921 | 0.15671024058573774 | |||
20100720 | 0.125 | 0.625 | 0 | 0 | 0.0303435976599782 | 0.2753822925732928 | 0.1101871778916296 | 0.17622331436830277 | 0.03575704541999343 | 0.3921 | 0.27738814539306944 | |
20100720 | 0.125 | 0.625 | 0 | 0 | 0.0641678314228708 | 0.2753822925732928 | 0.2330136437722937 | 0.3921 | 0.5865947720418742 | |||
20100720 | 0.125 | 0.625 | 0 | 0 | 0.0510749118639729 | 0.2753822925732928 | 0.185469121440985 | 0.3921 | 0.4669049213221114 | |||
20100720 | 0.125 | 0.3125 | 0 | 0 | 0.00754441810776365 | 0.2753822925732928 | 0.027396162757108683 | 0.06619170309306861 | 0.02591068181677811 | 0.3921 | 0.06896783204262777 | |
20100720 | 0.125 | 0.3125 | 0 | 0 | 0.0153762426196316 | 0.2753822925732928 | 0.05583598885734174 | 0.3921 | 0.14056301006782285 | |||
20100720 | 0.125 | 0.3125 | 0 | 0 | 0.0317634081139046 | 0.2753822925732928 | 0.11534295766475543 | 0.3921 | 0.29036744313612495 | |||
20100720 | 0.125 | 0.15625 | 0 | 0 | 0.0131288077769768 | 0.2753822925732928 | 0.047674843775521905 | 0.09309984979970691 | 0.028074756403237647 | 0.3921 | 0.12001792540510234 | |
20100720 | 0.125 | 0.15625 | 0 | 0 | 0.0240213489552974 | 0.2753822925732928 | 0.08722909788727298 | 0.3921 | 0.2195928614403623 | |||
20100720 | 0.125 | 0.15625 | 0 | 0 | 0.0397639934959433 | 0.2753822925732928 | 0.14439560773632584 | 0.3921 | 0.36350536059901456 | |||
20100720 | 0.125 | 0.0391 | 0 | 0 | 0.00813426178560675 | 0.2753822925732928 | 0.029538071273924858 | 0.056842058352765776 | 0.02730398707884092 | 0.3921 | 0.07435992976624496 | |
20100720 | 0.125 | 0.0391 | 0 | 0 | 0.0231723309019323 | 0.2753822925732928 | 0.0841460454316067 | 0.3921 | 0.2118315028214137 | |||
20100720 | 0.125 | 0.0195 | 0 | 0 | 0.0565283999793485 | 0.2753822925732928 | 0.2052724576120068 | 0.10383951516679084 | 0.05079137120415914 | 0.3921 | 0.5167583688664152 | |
20100720 | 0.125 | 0.0195 | 0 | 0 | 0.0159443319783087 | 0.2753822925732928 | 0.05789890057678683 | 0.3921 | 0.14575623914325386 | |||
20100720 | 0.125 | 0.0195 | 0 | 0 | 0.013313959281333 | 0.2753822925732928 | 0.04834718731157887 | 0.3921 | 0.12171050098515108 | |||
20100720 | 0.125 | 0.00977 | 0 | 0 | 0.050205488955729 | 0.2753822925732928 | 0.18231197251859196 | 0.11844799330499091 | 0.032388010192831364 | 0.3921 | 0.4589570302782583 | |
20100720 | 0.125 | 0.00977 | 0 | 0 | 0.0264081983300297 | 0.2753822925732928 | 0.09589650112670617 | 0.3921 | 0.24141241391428847 | |||
20100720 | 0.125 | 0.00977 | 0 | 0 | 0.0212417525553446 | 0.2753822925732928 | 0.07713550626967464 | 0.3921 | 0.19418298424109035 | |||
20100720 | 0.125 | 0.00488 | 0 | 0 | 0.053871590637829 | 0.2753822925732928 | 0.19562474454849388 | 0.12494435971445857 | 0.035908680415504345 | 0.3921 | 0.49247095825132187 | |
20100720 | 0.125 | 0.00488 | 0 | 0 | 0.0216397888263831 | 0.2753822925732928 | 0.0785809015684031 | 0.3921 | 0.19782166098139561 | |||
20100720 | 0.125 | 0.00488 | 0 | 0 | 0.0277110132025972 | 0.2753822925732928 | 0.10062743302647877 | 0.3921 | 0.2533221882706977 | |||
20100720 | 0.125 | 0 | 0 | 0 | 0 | 0.2753822925732928 | 0 | 0 | 0 | 0.3921 | 0 | |
20111109 | 10 | 5 | 0 | 0 | 0.036616368 | 0.11127965109742953 | 0.329048192 | 0.29244506681196564 | 0.019340009296955528 | 0.3921 | 0.8283547094162967 | |
20111109 | 10 | 5 | 0 | 0 | 0.031711636 | 0.11127965109742953 | 0.2849724607083398 | 0.3921 | 0.7173972859322196 | |||
20111109 | 10 | 5 | 0 | 0 | 0.029301551 | 0.11127965109742953 | 0.263314547727557 | 0.3921 | 0.6628750771800141 | |||
20100720 | 10 | 1.25 | 0 | 0 | 0.180774397889264 | 0.18348626141861277 | 0.9852203456085368 | 0.9856227951974149 | 0.01693008264438957 | 0.3921 | 2.480220019253541 | |
20100720 | 10 | 0.3125 | 0 | 0 | 0.08323247186823476 | 0.11127965151247686 | 0.747957697 | 0.78158444675 | 0.0271661560717674 | 0.3921 | 1.882928688920186 | |
10 | 0 | 0 | 0 | 0 | 0.11127965151247686 | 0 | 0 | 0 | 0.3921 | 0 |