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["Metadata","Values (examples)",null,null,"Notes"]
[]
["Asset Title ","Activity of TbTryS measured by the spectrophotometric assay",null,null,"The name of the data file"]
["Uploader","Alejandro Leroux",null,null,"The person submitting the asset to SEEK"]
["Uploader SEEK ID",346,null,null,"If you add your own SEEK ID, this will help us link this asset with your profile"]
["Project","SilicoTryp","","","The project that the asset belongs to"]
[]
["ASSAY"]
["Assay SEEK ID",194,null,null,"If referring to an exisitng Assay, you can link to it via the Assay SEEK ID."]
["Assay Title","Trypanothione synthetase ATP consumption steady state data",null,null,"The title of an exisitng assay"]
["Assay_type","continuousEnzymatic",null,null,"The assay_type describes the type of experiment you are performing"]
["Technology_type","enzymatic_activity_measurements","","","Describes the type of instruments and/or equipment used for the experiment"]
["Description","This assay is designed to obtain the in vitro kinetic data of T. brucei recombinant trypanothione synthetase. The enzyme catalyzes the ATP-dependent ligation of spermidine (Spd) and GSH to generate glutathionylspermidine (Gsp) and also of Gsp and GSH to finally produce trypanothione (T(SH)2). The data was obtained in an spectrophotometric assay that links ADP production with NADH consumption through the piruvate kinase and lactate dehydrogenase.",null,null,"A brief, human readable description."]
["Experimentalist","Alejandro Leroux",null,null,"The names of the people who carried out the experiments. These can either be SEEK members or external scientists"]
["Date","2012-07-20",null,null,"The start date for the experiment if different from the upload date"]
["SOP","https://seek.sysmo-db.org/sops/180","https://seek.sysmo-db.org/sops/181",null,"Links to SOPs and protocols used to carry out the experiment. If they are already in SEEK, you can refer to them by their SEEK ID"]
["Publication (optional)",null,null,null,"If this data appears in a publication, you can link it directly, or via the assay or study. If it is already registered in SEEK, you can use the PubMed ID or DOI as a reference."]
[]
["Experimental_conditions"]
["Item","pH","buffer","temperature","The name of the experimental condition you are fixing in your experiment (e.g. temperature, concentration, pH etc). If there is more than 1, please list them in columns across the spreadsheet"]
["Compound (if concentration)",null,"","","The compound name is only required if the item is concentration. "]
["Unit",null,null,null,"The SI units of the experimental conditions measurements. "]
["Start_value (optional)","",null,null,"This field is used for recording changes throughout the experiment to measure different conditions (e.g. pH or dilutions)"]
["End_value (optional)",null,null,null,"This field is used for recording changes throughout the experiment to measure different conditions (e.g. pH or dilutions)"]
["Comments",null,null,null,"Additional information that would be useful for people reading this data file"]
[]
["Culture growth","CultureGrowth"]
[]
["FACTORS_STUDIED"]
["Item","enzymeActivity","activity","activity","Factors Studied are the factors that you deliberately change during the course of your experiment to see the effects.\nFor example, if you measure the concentration of compounds at pH 5, 6 & 7, the factor studied is the pH (i.e. it is about the treatment you apply rather than the measurements you make). "]
["Compound (if concentration)",null,null,null,"The compound name is only required if the Factors Studied item is concentration. "]
["Unit",null,null,null,"The SI units of the factors studied measurements"]
["Start_value (optional)",null,null,null,"This field is used for recording changes throughout the experiment for one particular factor studied (e.g. pH in crease from 4 - 7 or dilution rate doubling)"]
["End_value (optional)",null,null,null,"This field is used for recording changes throughout the experiment for one particular factor studied (e.g. pH in crease from 4 - 7 or dilution rate doubling)"]
["SD (optional)",null,null,null,"The standard deviation for the difference in factors studied measurements (optional)"]
["Comments",null,null,null,"Additional information that would be useful for people reading this data file"]

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["<http://www.mygrid.org.uk/ontology/JERMOntology#2-hybrid_system>","2-hybrid_system"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#2D_gel_electrophoresis>","2D_gel_electrophoresis"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Accelerator_Mass_Spectrometry>","Accelerator_Mass_Spectrometry"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#affinity_chromatography>","affinity_chromatography"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Algebraic_equation>","Algebraic_equation"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#bait_protein_tagging>","bait_protein_tagging"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Batch>","Batch"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#binding>","binding"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#Chemostat>","Chemostat"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#chemostat_measurements>","chemostat_measurements"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#ChIP-on-chip>","ChIP-on-chip"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#chromatography>","chromatography"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#CultureGrowth>","CultureGrowth"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#custom_Array>","custom_Array"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#DNA_Methylation>","DNA_Methylation"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#electrophoresis>","electrophoresis"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#enzymatic_activity_measurements>","enzymatic_activity_measurements"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Exon_array>","Exon_array"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#FACS>","FACS"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#flow_cytometry>","flow_cytometry"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#FRAP>","FRAP"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#gas_chromatography>","gas_chromatography"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Gas_Chromatography_Mass_Spectrometry>","Gas_Chromatography_Mass_Spectrometry"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#gel_electrophoresis>","gel_electrophoresis"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#gel_filtration>","gel_filtration"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#HPLC>","HPLC"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#imaging>","imaging"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#initial_rate_experiment>","initial_rate_experiment"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Ion_Mobility_Spectrometry_Mass_Spectrometry>","Ion_Mobility_Spectrometry_Mass_Spectrometry"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#mathematical_modelType>","mathematical_modelType"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#microarray>","microarray"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#miRNA_microarray>","miRNA_microarray"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#molecule_labelling>","molecule_labelling"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#NMR>","NMR"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Ordinary_differential_equation>","Ordinary_differential_equation"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Partial_differential_equation>","Partial_differential_equation"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#PCR>","PCR"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#progressive_curve_experiment>","progressive_curve_experiment"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#qPCR>","qPCR"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#qRT-PCR>","qRT-PCR"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#radioactive_labelling>","radioactive_labelling"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#small_molecule_microarray>","small_molecule_microarray"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#SNP_array>","SNP_array"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#structural_methods>","structural_methods"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#surface_plasmon_resonance>","surface_plasmon_resonance"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Tandem_Mass_Spectrometry>","Tandem_Mass_Spectrometry"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#tiling_array>","tiling_array"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#UPLC>","UPLC"]

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["<http://www.mygrid.org.uk/ontology/JERMOntology#Chemostat>","Chemostat"]

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["<http://www.mygrid.org.uk/ontology/JERMOntology#intracellular_concentration>","intracellular_concentration"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#mass>","mass"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#optical_density>","optical_density"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#optical_density_600>","optical_density_600"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#pH>","pH"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#pressure>","pressure"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#specific_concentration>","specific_concentration"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#temperature>","temperature"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#volume>","volume"]

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["<http://www.mygrid.org.uk/ontology/JERMOntology#KOSMOBAC>","KOSMOBAC"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#MOSES>","MOSES"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Noisy-Strep>","Noisy-Strep"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#PSYSMO>","PSYSMO"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#ScaRAB>","ScaRAB"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#SilicoTryp>","SilicoTryp"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#STREAM>","STREAM"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#SulfoSys>","SulfoSys"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#SysmoLab>","SysmoLab"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Translucent>","Translucent"]

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["<http://www.mygrid.org.uk/ontology/JERMOntology#pH>","pH"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#pressure>","pressure"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#specific_concentration>","specific_concentration"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#temperature>","temperature"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#volume>","volume"]

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["<http://www.mygrid.org.uk/ontology/JERMOntology#Clostridium_acetobutylicum>","Clostridium_acetobutylicum"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Enterococcus_faecalis>","Enterococcus_faecalis"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Escherichia_coli>","Escherichia_coli"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Lactococcus_lactis>","Lactococcus_lactis"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Pseudomonas_fluorescens>","Pseudomonas_fluorescens"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Pseudomonas_putida>","Pseudomonas_putida"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Saccharomyces_cerevisiae>","Saccharomyces_cerevisiae"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Streptococcus_pneumoniae>","Streptococcus_pneumoniae"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Streptococcus_pyogenes>","Streptococcus_pyogenes"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Streptomyces_coelicolor>","Streptomyces_coelicolor"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Sulfolobus_solfataricus>","Sulfolobus_solfataricus"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Trypanosoma_brucei>","Trypanosoma_brucei"]

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["<http://www.mygrid.org.uk/ontology/JERMOntology#discontinuousEnzymatic>","discontinuousEnzymatic"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#Extracellular_Metabolite_concentration>","Extracellular_Metabolite_concentration"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#Metabolite_concentration>","Metabolite_concentration"]
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["<http://www.mygrid.org.uk/ontology/JERMOntology#methylation_profiling>","methylation_profiling"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#microRNA_profiling>","microRNA_profiling"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#organism_or_strain_characterisation>","organism_or_strain_characterisation"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#post-translational_modification>","post-translational_modification"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#primer_design>","primer_design"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#protein-protein_interaction>","protein-protein_interaction"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#protein-protein_interaction_profiling>","protein-protein_interaction_profiling"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#protein_expression_profiling>","protein_expression_profiling"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#protein_identification>","protein_identification"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#protein_purification>","protein_purification"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#protein_structural_analysis>","protein_structural_analysis"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#proteomics>","proteomics"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#reactomics>","reactomics"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#RNAi>","RNAi"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#single_cell>","single_cell"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#small-molecule_microarray>","small-molecule_microarray"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#SNP_detection>","SNP_detection"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#Substrate_utilization>","Substrate_utilization"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#tiling_path>","tiling_path"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#transcripomics>","transcripomics"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#transcriptional_profiling>","transcriptional_profiling"]
["<http://www.mygrid.org.uk/ontology/JERMOntology#translational_profiling>","translational_profiling"]

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["TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. ATP stock solution contained each 100 mM ATP and MgCl2. All assays were performed in a final volume of 0.2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 0.4 units pyruvate kinase, 0.4 units L-lactate dehydrogenase, 0.1-1 µM TbTryS, and varying amounts of ATP, GSH, and either spermidine or N-glutathionylspermidine. "]
[]
[]
["abreviation","Full name","ChEBI ID "]
["Spd","Spermidine",16610]
["Gsp","N-glutathionylspermidine",16613]
["ATP","Adenosine-5'-triphosphate ",15422]
["GSH","Glutathione",16856]
["T(SH)2","Trypanothione",17842]

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[null,"TbTryS Stock solution",null,null,"Assay"]
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["Unit","mg/mL","µM","µl","µl","µM","µM","µM","µM","µM","µM","AU/min","µmol/min/ml","µmol/min/mg","s-1","µM/s"]
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[null,"TbTryS Stock solution",null,null,"Assay"]
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[null,"TbTryS Stock solution",null,null,"Assay"]
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