Short Name: 04_stages-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR of stages samples Description: qPCR of stages samples for target genes pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station and manual Reaction volume: 5 ul qPCR platform: HT7900 Fast, ABI (SDS); Viia7, ABI (QuantStudio); LightCycler 480, Roche (LightCycler 480) qPCR protocol (file path, link or citation): https://dx.doi.org/10.3389%2Ffpls.2020.01250 One-step RT-PCR (RNA as input): No Well format: 384 MasterMix: FastStart Universal Probe Master (ROX), Roche (4914058001); TaqMan Universal PCR Master mix, ABI (4318157); Power SYBR® Green PCR Master Mix, ABI (4367659) No. sample technical replicates: 2 No. sample dilutions: 2 PCR controls used: NTC only No. standard curve dilutions (if using): 5 Operator: Marko Petek, Katja Stare Cq value determination software (if different from PCR platform): Quantification software: quantGenius qPCR quantification method: relative standard curve Notes:

SEEK ID: https://fairdomhub.org/assays/1573

Experimental Assay

Marko Petek

Projects: _p_RNAinVAL

Investigation: _I_01_LabTrials

Study: _S_04_Stages

Assay position:

Assay type: Experimental Assay Type

Technology type: Technology Type

Organisms: No organisms