SOPs

What is a SOP?
7 SOPs visible to you, out of a total of 8
Preparation of cell extracts of recombinant E.coli

Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.

Creators: Jacky Snoep, Theresa Kouril

Submitter: Jacky Snoep

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Cloning and heterologous expression in E. coli

Cloning and heterologous expression of gluconeogenic enzymes from S. solfataricus in E. coli

Creators: Jacky Snoep, Theresa Kouril

Submitter: Jacky Snoep

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Protein purification from E. coli extracts

Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts

Creators: Jacky Snoep, Theresa Kouril

Submitter: Jacky Snoep

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Sulfosys - SOP fermentation

SOPs related with medium composition, fermentation and stocking of S. solfataricus.

Creator: Pawel Sierocinski

Submitter: Pawel Sierocinski

Sulfosys - SOP metabolomics

Standard procedure regarding intracellular metabolome analysis using GC-MS.

Creator: Pawel Sierocinski

Submitter: Pawel Sierocinski

Sulfosys - SOP microarray

Protocol for RNA isolation, cDNA synthesis and labeling and hybridization and cleanup of Sulfolobus solfataricus microarray

Creator: Pawel Sierocinski

Submitter: Pawel Sierocinski

Sulfosys - SOP proteomics

Standard operating procedures regarding iTraq based proteomics.

Creator: Pawel Sierocinski

Submitter: Pawel Sierocinski

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