Assays

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349 Assays visible to you, out of a total of 372

Wenden_qPCR_clock_cFR, WT and phyA

Property Value
BioDare ID 13395774721132
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'Light inputs shape the Arabidopsis circadian system' by: Benedicte Wenden.

Experiments performed on phyA and Col-0, 22 March to 26 Aug 2009, and on elf4, 8 March through November 2010. Published in Wenden et al. Plant Journal 2011. Curated ...

CCA1 and CCR2 markers in elf4 mutant under Far Red light

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BioDare ID 12725469475452
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the CCA1 and CCR2 ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Unpublished

Clock genes under Far Red light - 6

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BioDare ID 12730680847492
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of CCA1 and CCR2 in phyb, elf3-4, elf4-1 and ...

Clock genes under Far Red light - 5

Property Value
BioDare ID 12725464084711
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the main clock markers in elf4, elf3, gi ...

Clock genes under Far Red light - 3

Property Value
BioDare ID 12723746109930
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the main clock markers under strict FR ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Unpublished

080115H1

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BioDare ID 2629
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

080115H2

Property Value
BioDare ID 2630
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Millar Andrew

Study: TiMet shady nights

210814H2

Property Value
BioDare ID 1926
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x7 light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

Partner experiment: 210814H1

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Millar Andrew

Study: TiMet shady nights

210814H1

Property Value
BioDare ID 1925
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the ...

190614H1

Property Value
BioDare ID 1642
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

190614H2

Property Value
BioDare ID 1643
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Millar Andrew

Study: TiMet shady nights

270313H1: 21L 7Dx4 LLx3 (T-28)

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BioDare ID 13649842953637
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 21L 7D Experimental conditions: 21L7Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime during the first three imaging days appear to be absent, ...

200313H2: 7L 21Dx4 LLx3 (T-28)

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BioDare ID 13643892291642
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 7L 21D Experimental conditions: 7L21Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime on imaging day 2 and day 3 appeared to be absent, using the ...

290313H2: 15L 5Dx4 LLx3

Property Value
BioDare ID 13649886952409
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 15L 5D Experimental conditions: 15L 5Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime during the first three imaging days appear to be absent, using the ...

270313H3: 12L-12Dx4 LLx3

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BioDare ID 13650039169340
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')

Purpose

'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments

Comments

200313H3: 12L-12Dx4 LLx3

Property Value
BioDare ID 13644021710933
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')

Purpose

'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments

Comments

060213H3: 12B-12Dx4 BBx3

Property Value
BioDare ID 13615365050841
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

Imaging script started at ZT+7mins, therefore ...

030413H3: 12B-12Dx4 BB3

Property Value
BioDare ID 13661271159675
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

AT0048 4x 18:6-DD

Property Value
BioDare ID 12730752301671
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

4 days of 18hrs light, 6hrs dark into 3 days constant dark

Purpose

changes to photoperiod

Comments

BW-detached_LL-LL_#14

Property Value
BioDare ID 13394382079593
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 14 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-detached_LL-LL_#16

Property Value
BioDare ID 13394383188744
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 16 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-detached_LD-LL_#06

Property Value
BioDare ID 13394381111502
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Detached leaves of CCA1:LUC grown in LD and imaged under constant light. Sample number 06 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-detached_LD-LL_#03

Property Value
BioDare ID 13394379875121
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Detached leaves of CCA1:LUC grown in LD and imaged under constant light. Sample number 03 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

BW-intact_LL-LL_#56

Property Value
BioDare ID 13394446929060
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 56 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-intact_LL-LL_#46

Property Value
BioDare ID 13394446784669
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 46 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-intact_LL-LD_#31

Property Value
BioDare ID 13394443003897
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Intact leaves of CCA1:LUC grown under constant light and imaged under LD. Plant number 31 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-intact_LL-LL_#37

Property Value
BioDare ID 13394444364628
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 37 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-intact_LL-LL_#58

Property Value
BioDare ID 13394446996851
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 58 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

BW-intact_LD-LL_#23

Property Value
BioDare ID 13394434885385
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Intact leaves of CCA1:LUC grown under LD and imaged under constant light. Sample number 23 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Wenden et al. (2012)

SDtoLD 260410

Property Value
BioDare ID 13982031569136
Author Laura Dixon
Institution University of Edinburgh
License CC_BY

Description

pTOC plate 0683 pCAB plate0684 LHY plate 0685 control plate 0686 TOC plate0687 pLHY plate 0688

Purpose

Re-entrainment dynamics during the transition between light:dark cycles with different photoperiods, comparing Arabidopsis thaliana and Ostreococcus tauri. This expt in O. tauri ...

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